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PLASMID BIOLOGY, SEPTEMBER 23-27, 2006
FALLEN LEAF LAKE, SOUTH LAKE TAHOE, CALIFORNIA
SCHEDULE - PLASMID BIOLOGY 2006
Saturday, September 23, 2006
13:00-20:00 Arrival and Registration at Main Lodge Lobby
17:00-18:00 Reception at lower level lounge, Bar and Deck
18:00-20:00 Dinner in dining room
20:00-20:15 Welcome Address: Neal Van Alfen, Dean of College of
Agricultural and Environmental Sciences
20:15-20:30 Opening of Plasmid Biology 2006: Donald R. Helinski and
Clarence I. Kado
20:30-21:20 Keynote address: Stanley N. Cohen “Bacterial plasmids: their extraordinary contributions to molecular biology”
21:30-00:00 Non-host bar and social
Sunday, September 24
07:30-08:30 Breakfast
Session I REPLICATION
Chairs: Chris D. Thomas and Manuel Espinosa
08:30-08:50 Troy Bankhead, Kerri Kobryn, and George Chaconas
“Unexpected twist: harnessing the energy in positive supercoils to control the telomere resolution step of B. burgdorferi DNA replication”
08:50-09:10 Deepak Bastia, Bidyut K. Mohanty and N.K. Bairwa
“Cell cycle master kinases CDK and DDK and the checkpoint proteins Tof1/Csm3 control replication termination and plasmid formation from the rDNA repeats of yeast”
09:10-09:30 Tateo Itoh “The mechanism of initiation of DNA replication in plasmids of the ColE2 family”
09:30-09:50 Lisa M. Bowers, Ricardo Krüger, and Marcin Filutowicz
“The activators of γ origin, monomers of π protein, bind iterons cooperatively”
09:50-10:10 Coffee and Tea break
10:10-10:30 Christopher D. Thomas and Catherine M. Joce
“The inverted complementary repeat is a consequence of, not a requirement for, the action of a replication initiator protein at a replication origin”
10:30-10:50 Eowyn Tinsley and Saleem A. Khan
“A novel FtsZ-like protein is involved in the replication of the anthrax toxin-encoding pXO1 plasmid in Bacillus anthracis”
10:50-11:10 José A. Ruiz-Masó, Rudi Lurz, Manuel Espinosa and Gloria del Solar “The double-strand origin of plasmid pMV158 revisited: RepB initiator interacts with two distinct DNA regions”
11:10-11:30 Wenke Zhang, Mark S. Dillingham, Christopher D. Thomas, Stephanie Allen, Clive J. Roberts and Panos Soultanas
“Directional loading of the PcrA helicase at the plasmid replication origin oriD by RepD and unwinding of a plasmid”
11:30-11:50 Monika Witosinska, Katarzyna Kolatka and Igor Konieczny
“Plasmid RK2 and its minireplicon derivatives localization in symmetrically and asymmetrically dividing bacterial cells”
11:50-12:10 Stephen M. Kwong, Ronald A. Skurray, and Neville Firth
“Antisense RNA-mediated replication control of Staphylococcal multiresistance plasmid psk41”
12:15-13:15 Lunch
13:30-17:00 POSTERS I
Chair: Anne-Laure Moyne*
17:00-18:30 Reception (Old Lodge and Deck)
18:30-20:00 Dinner
Session II PARTITION AND STABILITY
Chairs: Kenn Gerdes and Stuart Austin
20:00-20:10 Stuart Austin (overview of field)
20:10-21:00 Kurt Nordström (EMBO Lecturer)
“Past, present and future of plasmid biology”
21:00-21:20 Barbara Funnell , Anthony Vecchiarelli, Maria A. Schumacher, and Jennifer Surtees “P1 ParB architecture on single and paired pars partition sites”
21:20-21:40 Sota Hiraga and Shun Adachi
“Partition mechanism of F plasmid as a reaction diffusion
system”
21:40-21:50 Coffee & Tea Break
21:50-22:10 Nelly Dubarry, Franck Pasta and David Lane
“Role of ParABS systems in partition of Burkholderia cenocepacia chromosomes”
22:10-22:30 F. Pratto, A. I. Cicek, W. I. Weihofen, R. Lurz, W. Saenger, and Juan Alonso “Partioning proteins delta (ParA) and omega (ParB) reveal mechanism that contributes to Streptococcus pyogenes pSM19035 segregation”
22:30-22:50 Preeti Srivastava and Dhruba Chattoraj
“Segregation of Vibrio cholerae chromosomes at different growth rates”
23:00-01:00 no host bar
Monday, September 25
07:30-08:30 Breakfast in dining room
Session III TRANSFER I
Chairs: Fernando de la Cruz and Laura Frost
08:30-08:50 Joel F. Schildbach, Sarah L. Williams, Chris Larkin, Lubomir Dostal “Molecular analysis of F TraI function”
08:50-09:10 Carolina Elvira César, Fernando de la Cruz, and Matxalen Llosa
“Characterisation of the site-specific recombinase activity of conjugative relaxase TrwC”
09:10-09:30 Renee Tsolis
“How do the Brucella VirB proteins function during infection?”
09:30-09:50 Beth Traxler, Eliora Gachelet, and Rembrandt Haft
“Functional analysis of the F plasmid coupling protein TraD within the E. coli cell envelope”
09:50-10:10 coffee and tea break
10:10-10:30 Kelly Baptista and Diane E. Taylor
“Characterization of the transfer proteins of the temperature sensitive IncH1 plasmid R27”
10:30-10:50 Jens Reuther, Cordula Gekeler, Yvonne Tiffert, Jutta Vogelmann, and Gunther Muth “Conjugal DNA transfer by the FtsK-like TraB protein in antibiotic-producing Streptomycetes”
10:50-11:10 Geraldine Van der Auwera and Jacques Mahillon
“Transcriptome of conjugative plasmid pAW63 from Bacillus thuringiensis”
11:10-11:30 Steve W. Matson, Debi Haisch, Ernesto Villareal, Jun-Hyuk Choi, and Heather Ragonese “The F plasmid-encoded TraM protein stimulates rexlasosome mediated cleavage at oriT through an interaction with TraI”
11:30-11:50 Isabella C.Y. Lau, Tracy L. Raivio, and Laura Frost
“The Cpx envelope stress response destabilizes the F plasmid transfer
activator, TraJ, via the HSLVU protease in Escherichia coli”
12:10-13:10 Lunch
13:30-17:00 POSTERS II
Chair: Anne-Laure Moyne
17:00-18:30 Reception
18:30-20:00 Dinner
Session IV TRANSFER II
Chairs: Laura Frost and Ellen Zechner
20:00-20:20 Elisabeth Grohmann, Mohammad Yaser Abajy, Cem Söllü, Ertugrul-Kaan Celik, Karsten Arends, Jolanta Kopec, Walter Keller, Guenther Koraimann “Model for a type IV secretion-like system required for plasmid transfer in Gram-positive pathogens”
20:20-20:40 Peter J. Christie, Krishnamohan Atmakuri, Simon Jakubowski, Vidhya Krishnamoorthy, Eric Cascales, Richard Bayliss, and Gabriel Waksman
“Requirements for receptor binding and DNA translocation through the A. tumefaciens VirB/D4 type IV secretion system”
20:40-21:10 Eva M. Camacho, Ana Serna, and Josep Casadesus
“Regulation of conjugal transfer by DNA adenine methylation in the virulence plasmid of Salmonella enterica”
21:10-21:30 Monika Nuk, Andreas Reisner and Ellen Zechner
“Recombinase-based expression technology detects activation of plasmid promoters”
21:30-21:40 coffee and tea break
21:40-22:00 Yuqing Chen and Gary M. Dunny “Comparative Analysis of the Conjugative DNA Processing Systems of the Enterococcus faecalis Plasmid pCF10 and the Lactococcus lactis Plasmid pRS01”
22:00-22:20 M. Pilar Garcillán-Barcia, Blanca González, María Lucas, Paola Jurado, Gabriel Moncalián, Luis A. Fernández and Fernando de la Cruz
“Analysis of the termination reaction in conjugative DNA processing catalyzed by plasmid R388 relaxase TrwC”
Tuesday, September 26
07:30-08:30 Breakfast
Session V VIRULENCE AND PATHOGENICITY
Chairs: Steve Winans and Steve Farrand
08:30-09:00 Stephen K. Farrand, John S. Reader and Ingyu Hwang
“Rhizosphere Wars: A Tale of Three Plasmids”
09:00-09:20 Richard Novick and Carles Ubeda
"Comparative genetics and replication behavior of the
super-antigen-carrying staphylococcal pathogenicity islands"
09:20-09:40 Joseph P. Vogel “The adapted conjugation system of Legionella pneumophila”
09:40-10:10 Yasunori Machida, Shinji Terakura, Hideaki Tagami, Saeko Kitakura, Yoshihisa Ueno, and Chiyoko Machida
“An oncoprotein from the plant pathogen Agrobacterium has histone-chaperone activity”
10:10-10:30 coffee and tea break
10:30-10:50 Jorge H. Crosa “The pJM1 plasmid is essential in iron transport and
microbial virulence”
10:50-11:10 Glenn Young
“Transcriptional regulation of virulence traits in pathogenic Yersinia by the second messenger cAMP and the transcription factor CRP”
11:30-11:50 Adam Roberts** and Peter Mullhany “Characterization of a conjugative transposon from Enterococcus faecium; demonstration of the modular nature of mobile genetic elements”
12:10-13:30 lunch in dining room
Session VI EVOLUTION AND ECOLOGY
Chairs: David Romero and Eva Top
13:30-14:00 Christopher M. Thomas (USB Keynote Speaker), Anthony Haines, Lewis Bingle, Yanina Sevastsyanovich “Evolution of the IncP-1 and IncP-9 plasmids”
14:00-14:20 Stephen M. Krone, Ruinan Lu, Randal Fox, and Eva M. Top
“Modeling the spatial dynamics of plasmid transfer and persistence”
14:20-14:40 Hope McCaslin, Tsukasa Ito, Satoshi Okabe, Winnie DeJonghe, Joao de Bivar Xavier, and Stefan Wuertz “Bioaugmentation via conjugation in biofilms treating 3-chloroaniline: effects of selective pressure”
14:40-15:10 David Romero, Alejandro García-de los Santos and Susana Brom. “Essential plasmids in Rhizobium”
15:10-15:30 coffee and tea break
15:30-15:50 Wesley Loftie-Eaton, and Douglas Rawlings
“Insights into the evolution of IncQ plasmids derived from studies on pRAS3”
15:50-16:10 Masahiro Sota**, Eva M. Top, and Masataka Tsuda “Genomic and functional analysis of catabolic plasmids and transposons”
16:10-16:30 Eva M. Top, Leen De Gelder and Julia Williams. “Adaptive plasmid evolution improves the stability of a broad-host-range plasmid in an unfavorable host”
16:30-18:30 Grand reception (California wine tasting) Old Lodge Deck
18:30-20:30 Banquet
20:30 on Social and no host bar
Wednesday, September 27
07:30-08:30 Breakfast
Session VII NOVEL PLASMIDS AND USES IN
BIOTECHNOLOGY AND BIOINFORMATICS
Chairs: Saleem Khan and M. Gabriela Kramer
08:30-09:00 Maider Zabala, Christine Perret, Juan José Lasarte, Jesús Prieto and M. Gabriela Kramer “Gene Therapy of Cancer Using Plasmid-Based Vectors”
09:00-09:20 Joy Wireman and Anne Summers “Update on progress in plasmid genomics and bioinformatics”
09:20-09:40 Hideki Suzuki, Miguel A. Dominguez, Jennifer L. Wendt, Marcin Filutowicz, Li-Ke He, Andrea Szilagyi, Richard L. Gamelli, and Ravi Shankar “Novel strategy to treat multi-drug-resistant Gram-negative pathogens using bacterial conjugation”
09:40-10:00 Maria Kornienko, Allison Montalvo, Michael Lenard, Pravien Abeywickrema, Dawn L. Hall, Paul L. Darke
"Protein expression plasmids produced rapidly: streamlining cloning protocols and robotic handling" in assay and drug development technologies”
10:00-10:30 Check out of room
10:30-10:50 Ronald Godiska, Vinay Dhodda, Valerie Gilbert, Nikolai Ravin, and David Mead “Novel linear vector for cloning “unclonable” DNAs and constructing dual-insert libraries”
10:50-11:10 Robin Wong, Miriam Land, and Cliff S. Han
“The story told by 100 shotgun sequenced microbial genomes -- Why the clonability for genomes are different”
11:10-11:30 Ananda M. Chakrabarty “Bacterial plasmid in potential cancer therapy”
11:30-12:00 Chris M. Thomas (Brian Wilkins Prize awardees; conference summary, future prospects and venue)
12:00-13:00 Lunch
13:00 Farewell and Departure
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*NSF sponsored minority recipient
**Brian Wilkins prize recipient
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